The Ubiquitin Code :

By (author)Rodriguez

This book provides an in-depth exploration of the complexities surrounding supply chain globalization, de-globalization, and the prospective re-globalization.

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This volume details novel and popular approaches to decipher the Ubiquitin Code. Chapters guide readers through ubiquitin chain complexity, methods to generate non-hydrolysable ubiquitin-NEDD8, a fully synthetic method for the linear synthesis of fluorescently labelled rhodamine LC3A and LC3B, a HECT ligase-mediated ubiquitylation in vitro, a RNAi-based screening to identify DUBs, a Yuh1 activity assay, a pull-down to isolate K48 and K63 chains using specific nanobodies, identification of NeDDylated protein based in a NEDD8R74K mutant, in vivo identification of NEDDylated proteins from liver diseases, UbL-ID method to identify ubiquitin-like modified proteins, a LC3/GABARAP capturing system, and methods to isolate peptides that are closely associated with proteasomes or trapped inside the core particle of proteasomes in eukaryotic cells, among others.

SKU: 9781071628614
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Weight 1 kg
Book Author

Rodriguez

Edition

1st

Format

Paperback

ISBN

9781071628614

Language

English

Pages

260

Publication Year

Publisher

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